Services providers, retailers, and leisure and entertainment venues will have to ensure that at least 1-meter distances are maintained between people, to provide hand sanitizer for customers to use, and to regularly disinfect and air premises.
People displaying symptoms, such as cough, sneezing and difficulty breathing, will not be allowed in such places.
Wearing facemasks will no longer be mandatory for either visitors or the staff, but still recommended.
The Health Ministry confirmed that the same requirements will apply in public transport.
Lithuania is on Wednesday lifting the nationwide quarantine put in place on March 16 in a bid to stem the spread of the coronavirus, but is extending a state of emergency declared in late February.