Embraer 190 aircraft will operate the route up to 12 times per week.
LOT CEO Krzysztof Moczulski said in early August that the company was observing a growing need for international travel.
The Polish carrier launched Vilnius-London City flights on May 1, 2019 after winning a tender procedure to operate the route under a special state aid mechanism.
Under the latest recommendations, while 14-day self-isolation is not required for Lithuanians after entering the United Kingdom, it is mandatory for travelers from the UK on arrival in Lithuania.